The AceQ Universal SYBR qPCR Master Mix delivers high-sensitivity quantitative PCR with superb specificity. Optimized for all qPCR instruments, it ensures accurate and reliable results for various molecular biology applications.
Available formats: 500 rxn or 2500 rxn
Price:
Send Request
The AceQ Universal SYBR qPCR Master Mix from Vazyme is a specialized premix designed for quantitative PCR (qPCR) utilizing the SYBR Green I chimeric fluorescence method.
At its core, this master mix features AceTaq DNA Polymerase, a chemically modified hot-start DNA polymerase. This innovative enzyme, combined with an optimally designed buffer, effectively inhibits non-specific amplification, significantly boosting overall amplification efficiency. This makes the AceQ Universal SYBR qPCR Master Mix ideal for high-sensitivity qPCR applications.
This product is a convenient 2× premixed reagent containing the ideal concentration of SYBR Green I for qPCR reactions. It enables the generation of robust standard curves across a broad quantitative range, allowing for precise quantification and detection of target genes with excellent repeatability and high reliability.
A key advantage of this master mix is its inclusion of a unique ROX Passive Reference Dye. This makes it universally compatible with all qPCR instruments, eliminating the need to adjust ROX concentration on different platforms. Simply add your primers and templates to the reaction system to initiate amplification.
Key Features & Advantages:
- Universal ROX Passive Reference Dye: Compatible with all qPCR instruments, requiring no ROX concentration adjustment.
- Rigorous Hot-Start DNA Polymerase: Features chemically modified hot-start AceTaq DNA Polymerase for strong amplification performance and high specificity.
- Optimized Reaction System: Patented buffer formula minimizes non-specific amplification and primer dimerization without requiring repeated optimization of conditions.
- Excellent Amplification Performance: Delivers repeatable and reliable quantitative results, meeting the stringent data requirements of high-impact publications.
Established Applications:
The AceQ Universal SYBR qPCR Master Mix is widely used in various research and diagnostic settings, including:
- Gene Expression Analysis: Accurately quantify gene expression levels, providing insights into cellular processes and biological pathways.
- Pathogen Detection: Efficiently detect and quantify microbial agents, crucial for infectious disease diagnosis and food safety.
- Genetic Variation Studies (e.g., Genotyping): Investigate genetic variants, useful in basic scientific research, medicine, and agriculture.
