For a Convenient, Easy & Fast method for Apoptosis Detection by Flow Cytometry and Fluorescence Microscopy

Annexin V Apoptosis Detection Kits are a convenient, easy-to-use and safe method for Apoptosis Detection. Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS).

Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry.

Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.

• Easy and one step protocol: get results in 9 minutes.
• Detection Method: Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy.
• Mammalian Reactivity.
• Proven performance for both adherent and suspension cells.
• Versatile: most used kits availables.
• Safe: non-enzymatic assay that avoids fixation.
• Risk-free: product covered by our Quality 100% Guarantee.

References

• STAUROSPORINE-INDUCED APOPTOSIS: ANALYSIS BY DIFFERENT ANNEXIN V ASSAYS. Slovak J. Anim. Sci., 52, 2019 (2): 47–52
• Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells. Biology 2019.
• Peroxiredoxin 6 Down-Regulation Induces Metabolic Remodeling and Cell Cycle Arrest in HepG2 Cells. Antioxidants 2019.
• Cell-Free Coelomic Fluid Extracts of the Sea Urchin Arbacia lixula Impair Mitochondrial Potential and Cell Cycle Distribution and Stimulate Reactive Oxygen Species Production and Autophagic Activity in Triple-Negative MDA-MB231 Breast Cancer Cells. Journal of Marine Science and Engineering 2020.