Capping reagent A containing 10% acetic anhydride in tetrahydrofuran (90:10 v/v) ensures fast and efficient acetylation of unreacted 5’-hydroxyl groups in oligonucleotide synthesis.
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Capping Reagent A – 10% Acetic Anhydride in THF
Tetrahydrofuran : Acetic Anhydride (90:10 v/v)
This capping reagent A formulation consists of 10% (v/v) acetic anhydride in tetrahydrofuran (THF). It is used during the capping step in solid-phase oligonucleotide synthesis to acetylate unreacted 5’-hydroxyl groups and prevent formation of deletion sequences.
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Composition: THF : Acetic Anhydride (90:10 v/v)
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Application: Capping reagent A for oligonucleotide synthesis
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Function: Acetylation of unreacted 5’-OH groups
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Instrument Compatibility: Manual and automated DNA/RNA synthesizers
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Format: Ready-to-use
