CVX™ Viral RNA Extraction Kit (CE-IVD) is designed for the rapid simultaneous purification of viral RNA from cell –free samples such as Serum, Plasma, Urine, Cell free body fluids, Cell Culture supernatants and Rinse liquid from swabs samples.

Viral RNA molecules bind to the silica-based media and impurities such as proteins and nucleases are removed by thorough washing with Wash Buffer. The RNA is then eluted in sterile, RNase free water. The isolated Viral RNA is ready-to-use and should be stored at – 70 °C.

The procedure can be used for isolation of viral RNA from a broad range of viruses. However, performance cannot be guaranteed for every virus species and must be validated by the customer. The amount of purified viral RNA depends on the sample type, the virus titer, sample source, transport, storage, and age. The Kit also includes carrier RNA that improves binding and recovery of low-concentrated viral RNA.

Advantages and Features

• Specifically validated for SARS-CoV-19 by Spanish Institution Instituto de Salud Carlos III (ISCIII)
• CE-IVD Certified for usage in in-vitro Diagnostics (License No.: 7817-PS).
• Compatible with Manual and Automated Extractions with Qiagen™ QIAcube™.
• Best performance: 100% Specificity and 98,3%  Sensitivity.
• Efficient.
• Fast results with minimal handling steps.
• Safe: no phenol-chloroform extraction.
• Ready-to-use: viral RNA is suitable for use in RT-PCR and qRT-PCR.

CVX™ Viral RNA Extraction Kit has been validated for RNA isolation from SARS-CoV-19 clinical samples. 60 positive samples and 20 negative samples were collected from nasopharyngeal exudates and RNA isolation was made in parallel using it and a comparator kit. After RNA isolation qPCR was done using the protocol described by Corman VM et al, 2020, also known as “Charité protocol”. An internal mRNA co-isolated along with viral RNA was used as a positive control.

CE-IVD Certified for usage in in-vitro Diagnostics (License No.: 7817-PS).