The Equalbit RNA High Sensitivity Kit is a simple, sensitive and accurate RNA fluorescence quantitative detection kit, which contains fluorescence detection reagents, buffers and RNA standards. The kit is highly selective for RNA over double-stranded DNA (dsDNA).

The kit has an excellent linearity for RNA samples in the range of 4 – 100 ng, allowing accurate quantification of total RNA, rRNA,  mRNA concentrations from 200 pg/μl to 200 ng/μl. In addition, it has good impurity tolerance to common contaminants, such as salts, free  nucleotides, proteins, solvents, detergents, etc.

This product is easy to operate and can be carried out at room temperature. Before use, please dilute the fluorescence detection reagent into a working solution with buffer and add the RNA sample into it. The assay result will  be obtained by the Qubit Fluorometer.

Features of the Equalbit RNA High Sensitivity Kit

• Sensitivity: It can accurately quantify total RNA, rRNA, and mRNA samples with a concentration of 250 pg/µl to 100 ng/µl.
• High specificity: This product is highly selective for RNA, not affected by DNA, and has good resistance to some conventional pollutants, such as salt, free nucleotides, proteins, solvents, detergents, etc. tolerance.
• Easy to operate: This product is easy to operate. Dilute the fluorescence detection reagent with buffer solution into a working solution, then add the RNA sample to be tested, and detect it with a Qubit fluorescence instrument. This operation can be carried out at room temperature.

Applications of High-Sensitivity RNA Quantification in Scientific Research: Accurate and sensitive RNA quantification is a critical initial step for numerous demanding molecular biology applications, especially when working with limited sample material. The Equalbit RNA HS Assay Kit is particularly valuable for:

1. Single-Cell RNA Sequencing (scRNA-Seq): Essential for quantifying the minute amounts of RNA obtained from individual cells, which is crucial for successful library preparation and subsequent transcriptomic analysis at a single-cell resolution.
2. Degraded or Challenging Samples: Ideal for RNA quantification from FFPE (Formalin-Fixed Paraffin-Embedded) tissues, liquid biopsies, or other samples where RNA yield is typically low or the RNA is fragmented.
3. MicroRNA and Non-coding RNA Studies: Due to the often low abundance of specific RNA species like microRNAs, a high-sensitivity assay is indispensable for accurate quantification before downstream applications such as specialized sequencing or RT-qPCR.

References

• Chemically modified tRNA enhances the translation capacity of mRNA rich in cognate codons. Nature Communications 2025.
• Plasma Biomarkers of Mitochondrial Dysfunction in Patients with Myasthenia Gravis. Medical Sciences 2025.