HiScript® III RT SuperMix for qPCR (+gDNA wiper) includes HiScript III Reverse Transcriptase, a new generation of reverse transcriptase.
The kit is applicable for reverse transcription reactions of animal, plant and microbial RNA. The RT product is compatible with dye-based and probe-based qPCR.
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HiScript III RT SuperMix for qPCR (+gDNA wiper)
This kit is an upgraded version of HiScript II Q RT SuperMix for qPCR (+gDNA wiper), including HiScript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer.
The HiScript III RT Supermix further improves upon the efficiency of cDNA synthesis established by HiScript II, and is suitable for two-step qRT-PCR detection.
The 4 × gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need of spanning an exon-exon junction; 5 × HiScript III qRT SuperMix contains all the components required for the reverse transcription reaction.
The reaction can be performed quickly by adding template RNA and RNase-free ddH2O, and at the same time, the gDNA wiper function is terminated to ensure the integrity of the cDNA.
The kit is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
Features of this Kit
- Effortless Workflow: Just add RNA to kickstart reverse transcription. Complete gDNA removal and cDNA synthesis in just 18 minutes!
- Broad Compatibility: Works seamlessly with various species and even degraded RNA, ensuring reliable results across different sample types.
- Unmatched Impurity Tolerance: Overcomes common reverse transcription inhibitors, including ethanol, isopropanol, phenol, guanidine isothiocyanate, and humic acid, for consistent performance.
- Superior cDNA Synthesis Efficiency: Achieve lower CT values in qPCR, proving its exceptional efficiency in generating high-quality cDNA.
