For highly Efficient, Accurate & Easy general Cloning with classical MCS, without the use of Toxic Genes

Product Overview:

pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases.

The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Advantages and Features

• Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
• Great efficiency: over hundreds positive colonies with few nanograms of insert.
• High stability: eliminates cloning bias or pitfalls.
• Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
• Powerful: clone from less than 1 ng/kb to up to 14 kb; obtain 4-fold more positive colonies using 3-fold less DNA insert.
• Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes.
• Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers.
• Flexible: ligation time from 10 minutes to overnight.
• Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
• High cost-saving:  reduces your cloning costs as no expensive phosphorylated primers are needed.
• Eliminates positive selection vector.
• Risk-free: product covered by our Quality 100% Guarantee.

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White Paper: Advantages of pSpark® over other popular DNA Cloning systems on the market: pGEM®-T and TOPO TA cloning®

References

• Molecular and biochemical analysis of XDH from Phaseolus vulgaris suggest that uric acid protects the enzyme against the inhibitory effects of nitric oxide in nodules. Plant Physiology and Biochemistry 2019.
• Identification and Molecular Characterization of Superoxide Dismutases Isolated From A Scuticociliate Parasite: Physiological Role in Oxidative Stress. Nature Scientific Reports 2019.
• A real-time quantitative PCR assay using hydrolysis probes for monitoring scuticociliate parasites in seawater. Aquaculture 2022.