High-performance rRNA depletion kit for bacterial RNA-seq applications. Removes 5S, 16S, and 23S rRNA efficiently to enrich for mRNA. Compatible with all major library prep protocols.
Kit size: 12- or 24-reactions.
Download the Ribo-off rRNA Removal Kit Bacteria Protocol (PDF)
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Ribo-off® rRNA Depletion Kit (Bacteria)
Efficient removal of bacterial ribosomal RNA for transcriptome research
Product Highlights:
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Broad Compatibility: Supports rRNA removal from Gram-negative and Gram-positive bacteria.
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Comprehensive Removal: Depletes 5S, 16S, and 23S rRNA.
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Magnetic Bead-Based Workflow: Simple, robust, and effective.
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Protocol Efficiency: Allows completion of depletion in under 2 hours.
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Flexible Input: Works with 10–1000 ng of total RNA.
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Universal Library Prep Compatibility: Downstream compatibility with Vazyme’s VAHTS RNA-seq kits and other brands.
Applications:
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RNA-seq and Transcriptomics: Improves detection sensitivity by reducing rRNA background.
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Microbial Gene Expression Studies: Accurate quantification of bacterial mRNA.
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Mixed Microbiome Samples: Applicable to both single strains and complex communities.
