Effective removal of cytoplasmic and mitochondrial rRNA from human, mouse, and rat RNA using RNase H technology. Boosts RNA-seq sensitivity and coverage.
24- or 96-reactions.
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Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) uses an RNase H-based approach to efficiently remove cytoplasmic (18S, 28S) and mitochondrial (12S, 16S) rRNA species from total RNA. This depletion maximizes the proportion of informative reads in RNA-seq libraries, improves detection of low-abundance transcripts, and enables comprehensive transcriptome analysis.
Suitable for a wide range of RNA inputs (100 ng–1 μg), the kit provides a fast, reliable workflow compatible with standard library preparation protocols.
Key Features:
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RNase H-based rRNA removal
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Targets cytoplasmic and mitochondrial rRNA
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Retains mRNA and non-coding RNAs
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Input: 100 ng–5 μg total RNA
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Rapid 2-hour protocol
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Consistent and unbiased depletion across samples
Applications:
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Human, mouse, and rat RNA-Seq
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Differential gene expression analysis
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Non-coding RNA discovery
