TBS with Tween 20 pH 7.4 (20X)

TBS (Tris-Buffered Saline) with Tween 20 is a commonly used buffer solution in biological and biochemical research. This specific formulation, at pH 7.4 and provided as a 20X concentrated stock solution, is designed for convenient and efficient laboratory use.

Key Features:

• Ready-to-Use Format: Supplied as a 20X concentrated solution, allowing for easy dilution to a 1X working solution.
• Optimal pH: Maintained at pH 7.4, which is physiologically relevant and suitable for a wide range of biological applications.
• High Quality: Manufactured under stringent quality control to ensure consistency and reliability in experimental results.

Applications:

TBS with Tween 20 is widely utilized in various laboratory procedures, particularly where washing steps are crucial to reduce non-specific binding and improve signal-to-noise ratio. Established applications include:

1. Western Blotting: Essential for washing membranes after antibody incubations to remove unbound antibodies, minimizing background noise and enhancing detection of specific proteins.
2. ELISA (Enzyme-Linked Immunosorbent Assay): Used as a wash buffer to remove unbound reagents, ensuring accurate quantification of analytes by reducing non-specific interactions.
3. Immunohistochemistry (IHC) and Immunofluorescence (IF): Employed for washing tissue sections or cells to eliminate excess antibodies and other reagents, which is critical for clear and specific signal visualization.

This buffer solution provides a stable environment for proteins and nucleic acids, making it an indispensable component in many molecular biology and immunology workflows.