This 10x TE Buffer pH 7.4 is a high-quality Tris-EDTA solution crucial for DNA/RNA protection. It prevents nucleic acid degradation by chelating metal ions and aids in antigen unmasking for enhanced staining.
Available formats: 1 L (1X), 1 L (10X), 5 L (10X)
We can also custom manufacture this product from Research-grade to Clinical-grade under GMP basis on commercial scale.
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TE Buffer 10x pH 7.4, a high-quality Tris-EDTA (TE) solution, is formulated with a robust buffer (Tris) and a potent chelating agent (EDTA). The EDTA component is vital for preventing the degradation of DNA and RNA by effectively chelating magnesium and other divalent metal ions, which are often cofactors for nucleases.
This 10x concentrated solution is generally used to solubilize nucleic acids, providing robust protection from enzymatic degradation, thereby preserving sample integrity. Beyond nucleic acid applications, TE Buffer 10x pH 7.4 is also valuable for its ability to break protein cross-links, facilitating the unmasking of antigens and epitopes. This unmasking significantly enhances the intensity of antibody staining in various immunological procedures.
Key applications of TE Buffer 10x pH 7.4 include:
- Nucleic Acid Protection and Solubilization: Essential for protecting and solubilizing DNA and RNA samples, ensuring their stability for downstream applications.
- Electrophoresis Buffering: Used as a running buffer in polyacrylamide and agarose gels for nucleic acid electrophoresis, supporting effective separation.
- Antigen/Epitope Unmasking: Valuable in immunohistochemistry and related techniques for breaking protein cross-links, thus enhancing antibody binding and staining.
