Ultramild capping reagent A is a 85:10:5 mixture of THF, pyridine, and phenoxyacetic anhydride, designed for gentle 5’-OH capping in sensitive oligonucleotide synthesis protocols.
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Capping Reagent A – Ultramild
Tetrahydrofuran : Pyridine : Phenoxyacetic Anhydride (85:10:5 v/v/v)
This reagent is a capping reagent A formulation for ultramild oligonucleotide synthesis, composed of tetrahydrofuran (THF), pyridine, and phenoxyacetic anhydride in a 85:10:5 volume ratio.
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Composition: THF : pyridine : phenoxyacetic anhydride (85:10:5, v/v/v)
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Application: Capping reagent A for ultramild DNA/RNA synthesis protocols
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Function: Acetylation of unreacted 5’-hydroxyl groups
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Compatibility: Ideal for oligonucleotides with labile modifications
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Format: Ready-to-use
This gentler alternative to traditional acetic anhydride–based formulations helps preserve sensitive base modifications or terminal labels. Suitable for both manual and automated synthesizers.
