CentriPure 200, a 20 mL Desalting Column – Gravity-Flow Gel Filtration for DNA, RNA, and Proteins

The CentriPure 200 desalting column is a pre-packed gravity-flow column that enables fast and efficient purification of biomolecules. It is designed for buffer exchange, removal of small molecules such as salts, dyes, or unreacted reagents from proteins, nucleic acids, and other macromolecules.

This column is perfect for medium research-scale, or production-scale purification of proteins and nucleic acids.

Features:

• Gravity-flow column

• Pre-packed with Zetadex® size-exclusion resin

• Molecular weight cutoff (MWCO): ~5 kDa (<10 bp for nucleic acids)

• Suitable for desalting and buffer exchange

• Ready-to-use format

Applications:

• Desalting of DNA, RNA, and proteins

• Removal of small molecules prior to labeling or enzymatic steps

• Buffer exchange in molecular biology workflows

• Sample cleanup for sensitive downstream reactions

Specifications:

• Column format: Z25M

• MWCO: ~5 kDa (nucleic acids <10 base pairs)

• Flow type: gravity – no centrifugation or vacuum needed

The CentriPure 200 desalting column is ideal for mid- to large-scale sample processing where consistent recovery, high purity, and speed are essential.

References

• An oligomeric state‐dependent switch in the ER enzyme FICD regulates AMPylation and deAMPylation of BiP. The EMBO Journal 2019.
• Linker length affects photostability of protein-targeted sensor of cellular microviscosity. Marketa Kubankova et al 2019 Methods Appl. Fluoresc. in press
• Current approaches for purification of antibody-drug conjugates. Journal of Separation Science 2021.