3% Trichloroacetic Acid in Methylene Chloride – Deblocking Reagent for Oligonucleotide Synthesis

This synthesis-grade solution of 3% trichloroacetic acid (TCA) in methylene chloride (also known as dichloromethane, DCM) is designed for the deblocking (detritylation) step in phosphoramidite oligonucleotide synthesis workflows. It efficiently removes the 5’-dimethoxytrityl (DMT) group to allow subsequent nucleotide coupling steps.

TCA is a widely used alternative to dichloroacetic acid in deblocking protocols, offering robust performance, rapid detritylation, and minimal side reactions when handled under controlled conditions.

Key Features:

• 3% (v/v) trichloroacetic acid in methylene chloride (DCM)

• Used for 5’-DMT deprotection in DNA/RNA synthesis

• High-purity, synthesis-grade formulation

• Compatible with manual and automated synthesizers

• Supplied in sealed, moisture-protected containers

Applications:

• Detritylation step in phosphoramidite oligonucleotide synthesis

• Removal of 5′-DMT groups during DNA/RNA manufacturing

• Custom oligo production requiring TCA-based protocols

Distribution:

• Available across Denmark, Sweden, Norway, and Iceland

• Fast delivery and technical support from YouDoBio