Capping B solution with 16% N-methylimidazole in tetrahydrofuran (84:16 v/v) for efficient 5’-OH acetylation during oligonucleotide synthesis. Ideal for automated platforms.
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Capping B Solution – 16% N-Methylimidazole in Tetrahydrofuran (84:16 v/v)
This Capping Reagent B is a ready-to-use solution composed of 16% N-methylimidazole in tetrahydrofuran (THF), mixed in an 84:16 volume ratio. It is designed for use in the capping step of solid-phase oligonucleotide synthesis, where it functions as an activator for acetic anhydride (Capping Reagent A).
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Composition: 84% THF / 16% N-methylimidazole (v/v)
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Function: Activator for Capping Reagent A
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Application: For 5’-OH acetylation during DNA/RNA synthesis
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Instrument Compatibility: Suitable for both manual and automated synthesizers
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Formulation: Ready-to-use
This formulation delivers a precise, controlled activation of the capping step and is often favored in automated or high-throughput synthesis systems due to its low viscosity and stability in THF.
