ClonExpress Ultra One Step Cloning Kit enables fast, ligase-free seamless cloning of 1–5 fragments (50 bp–10 kb) with >95% positive rate—streamlined workflow and high impurity tolerance.
Available in 25 or 50 reaction kit sizes.
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The ClonExpress Ultra One Step Cloning Kit from Vazyme Biotech represents a new generation of recombinant cloning solutions, designed to simplify and accelerate DNA manipulation workflows. This innovative kit employs a homologous recombination technology, allowing for the rapid and directional insertion of amplified DNA products into linearized vectors at virtually any site.
Key Features and Benefits:
- Simple and Fast: The streamlined one-step protocol significantly reduces hands-on time. Recombination can be completed in as little as 5 to 30 minutes at 50°C, making the cloning process exceptionally efficient.
- High Efficiency: With a consistently high positive rate, often exceeding 95%, the kit ensures successful cloning of fragments ranging from 50 bp to 10 kb. Its highly optimized 2 × CE Mix, containing enhanced recombinase Exnase, significantly boosts recombination efficiency and tolerance to impurities.
- Broad Compatibility: This versatile kit is compatible with 1 to 5 fragment homologous recombination, offering flexibility for various cloning workflows. It supports a wide range of vector types and fragment sizes, including those with challenging GC-rich or AT-rich homologous sequences (15-35 bp homology arms).
- Zero Background: The unique ligase-independent system minimizes self-ligation of the vector, virtually eliminating background issues and ensuring a high success rate. Restriction enzyme cutting sites on inserts do not need to be considered.
Established Applications:
The ClonExpress Ultra One Step Cloning Kit is widely utilized across various molecular biology disciplines:
- Plasmid Construction: Efficiently assemble new plasmids for gene expression, protein production, or other research purposes.
- Genetic Engineering: Facilitate precise and scarless insertion of genes or DNA fragments for advanced genetic manipulation experiments.
- Vector Construction: Streamline the process of creating various vectors, including those for gene therapy, vaccine development, or functional genomics studies
