Water-based formulations of Hoechst 33342 DNA stain.
We offer two formulations for flexibility – 20 mM (11.2 mg/ml) and 5 mg/ml – for fluorescent nuclear staining in live or fixed cells.
- F-0409, Hoechst 33342, 20 mM in water, 5 mL
- F-0411, Hoechst 33342, 5 mg/ml in water, 20 mL
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Product Overview
Hoechst 33342 is a cell-permeant blue fluorescent dye that binds to A-T rich regions of double-stranded DNA. It is widely used in nuclear staining applications, including fluorescence microscopy, flow cytometry, and live-cell imaging.
Available Formulations
1. Hoechst 33342 – 20 mM in Water
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~11.2 mg/ml concentration
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Highly concentrated stock for maximum dilution flexibility
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Ideal when minimal volume addition is preferred
2. Hoechst 33342 – 5 mg/ml in Water
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Moderate concentration
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Easier to pipette without ultra-low volume pipettes
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Suitable for protocols requiring lower stock solutions
Excitation/Emission
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Excitation: ~350 nm
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Emission: ~461 nm (blue fluorescence)
Applications
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Live-cell nuclear staining
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Fixed-cell imaging
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Flow cytometry
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Cell cycle and apoptosis assays
Hoechst 33342 is compatible with a wide range of cell types and is well-suited for live-cell experiments due to its membrane permeability and low cytotoxicity.
Choosing the Right Concentration
| Formulation | Concentration | Recommended Use |
|---|---|---|
| 20 mM in water | 11.2 mg/ml | High-concentration stock for flexible dilution and low-volume addition |
| 5 mg/ml in water | 5 mg/ml | Convenient for protocols requiring moderate stock concentration or larger pipetting volumes |
Protocol
Download the Hoechst_33342_Staining_Protocol (PDF) for detailed instructions on how to stain both live and fixed cells.
