HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.

Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.

• High specificity: minimizes unspecific amplification.
• Efficient: prevents or minimizes primer-dimer and nonspecific products.
• Great sensitivity: amplifies from femptograms of DNA targets.
• Inactive: at Room Temperature.
• Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
• Powerful: amplification of targets up to 5 kb.
• Convenient: available in kits and MasterMix solution.

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References

• Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain. European Journal of Plant Pathology 2019.
• Cadophora sabaouae sp. nov. and Phaeoacremonium species associated with Petri disease on grapevine propagation material and young grapevines in Algeria. Plant Disease 2021.