Magnetic bead total RNA purification kit for fast, phenol-free RNA extraction from cells, tissue, yeast or bacteria. Includes on-bead DNase I treatment. Automation compatible.
Available as 96- or 384-preps.
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The PuroMag™ Total RNA Purification Kit is a magnetic bead total RNA purification kit that simplifies the isolation of total RNA from a wide range of sample types, including:
- Bacteria
- Mammalian cells
- Yeast
- Animal tissue
This kit leverages a magnetic bead-based technology for nucleic acid binding, eliminating the need for hazardous phenol or chloroform. A crucial DNase I digestion step is included in the protocol to ensure the removal of any contaminating genomic DNA.
The flexible protocols allow for:
- Low-throughput manual purification using a magnetic rack.
- High-throughput automation on a variety of systems, including Auto-Pure Nucleic Acid Purification systems, Kingfisher Flex, Hamilton Microlab STAR, and Biomek FX.
The high-quality RNA purified with the PuroMag kit is suitable for a wide variety of downstream applications, such as:
- Reverse-transcription qPCR
- Northern blotting
- Microarray analysis
- cDNA library construction
Additionally, the kit can be used effectively for RNA cleanup after enzymatic treatments.
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