One-step Green RT-qPCR Kit High ROX enables combined cDNA synthesis and real-time PCR detection in a single tube with High ROX dye. Available in Denmark through You Do Bio.
Protocol for One-Step Green RT-qPCR Kit, High ROX (PDF)
Available in kit sizes of 100, or 500 reactions, based on a 20 µL reaction volume.
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One-step Green RT-qPCR Kit High ROX (qMAXSen™ One-Step Green RT-qPCR Kit, High ROX™) combines reverse transcription and quantitative PCR in a single tube, optimized for instruments that require a High ROX™ reference dye.
Features & Benefits:
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One-tube workflow reduces handling time and contamination risk.
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High sensitivity and specificity for SYBR Green-equivalent assays.
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Includes High ROX™ passive reference dye for signal normalization.
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Optimized for consistent performance across most High ROX™ instruments.
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Simple, reliable assay setup suitable for high-throughput use.
Kit Contents (for 500 × 20 µL reactions):
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5 × 500 µL (4×) One-Step Green RT-qPCR Mix
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5 × 100 µL RT Mix
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5 × 1 mL RNase-free Water
Applications (established uses):
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Gene expression analysis
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Quantitative detection of RNA targets
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Validation of RNAi or gene knockdown experiments
Usage Context:
This High ROX™ variant is recommended for real-time PCR platforms (such as ABI/Applied Biosystems systems) that require High ROX™ for baseline calibration.
