One-step SYBR Green RT-qPCR Kit Low ROX enables combined reverse transcription and qPCR in a single tube with Low ROX reference dye. Available in Denmark through You Do Bio.
Protocol for One-Step Green RT-qPCR Kit, low ROX (PDF)
Available in kit sizes of 100, or 500 reactions, based on a 20 µL reaction volume.
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One-step Green RT-qPCR Kit Low ROX (qMAXSen™ One-Step Green RT-qPCR Kit, Low ROX™) is designed for efficient reverse transcription and real-time PCR detection in a single reaction. This variant is optimized for instruments that require a Low ROX™ reference dye.
Features & Benefits:
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Combines cDNA synthesis and qPCR amplification in one tube.
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High sensitivity and specificity using SYBR Green – like chemistry.
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Includes Low ROX™ passive reference dye for signal normalization.
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Optimized for reproducibility across instruments requiring Low ROX™.
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Streamlined workflow reduces handling errors and contamination risk.
Kit Contents (for 500 × 20 µL reactions):
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5 × 500 µL (4×) One-Step Green RT-qPCR Mix
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5 × 100 µL RT Mix
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5 × 1 mL RNase-free Water
Applications (established uses):
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Gene expression analysis
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Quantification of RNA targets
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Validation of gene knockdown or RNAi experiments
Usage Context:
This Low ROX™ variant is best suited for real-time PCR instruments that require a Low ROX™ concentration for proper signal calibration (e.g., certain Applied Biosystems platforms).
