One-step RT-qPCR Kit High ROX ensures reliable cDNA synthesis and qPCR detection in a single tube with internal ROX reference dye. Available in Denmark through You Do Bio.
Protocol for One-Step Probe RT-qPCR Kit, high ROX (PDF)
Available in kit sizes of 100, or 500 reactions, based on a 20 µL reaction volume.
Price:
Send Request
One-step RT-qPCR Kit High ROX (qMAXSen™ One-Step Probe RT-qPCR Kit, High ROX™) provides efficient reverse transcription and real-time PCR in a single reaction, optimized for instruments requiring a High ROX™ reference dye.
Features & Benefits:
-
High sensitivity and specificity for probe-based detection.
-
Supports widely used probe chemistries: TaqMan®, Molecular Beacons®, Scorpion® probes.
-
Includes internal High ROX™ passive reference dye for normalization of fluorescence signal.
-
Streamlined one-tube workflow reduces handling and contamination risk.
-
Compatible with most real-time PCR platforms that require High ROX™.
Kit Contents (for 500 × 20 µL reactions):
-
5 × 500 µL (4×) One-Step Probe RT-qPCR Mix
-
5 × 100 µL RT Mix
-
5 × 1 mL RNase-free Water
Applications (established uses):
-
One-step RT-qPCR with sequence-specific probes
-
Quantitative detection of DNA and cDNA targets
-
Gene expression profiling
Usage Context:
Choose the High ROX™ version when working on real-time PCR platforms (such as ABI/Applied Biosystems instruments) that require a High ROX™ concentration for proper baseline calibration.
