PBS with Tween 20 (PBST) pH 7.4 Aqueous Solution, 1L Ready-to-Use

PBS with Tween 20 (PBST) pH 7.4 is a widely used wash buffer in various biological applications, particularly in immunoassays. This 1L ready-to-use format offers convenience and consistency for your laboratory needs.

Key Features:

• Ready-to-Use Solution: No preparation required, saving you valuable time and ensuring accuracy.
• Format: Aqueous solution, 1X and 10X formats.
• pH: 7.4 $\pm$ 0.05 at 25 °C.
• Volume: 1 L.
• Sterility: Sterile.
• Endotoxin: Endotoxin Tested ( < 0.25 EU/ml).
• RNase/DNase/Protease: Free.
• Storage: Store at room temperature (15-25 °C).
• Shelf Life: 24 months.

Applications:

PBS with Tween 20 (PBST) pH 7.4 is a versatile buffer solution critical in techniques requiring reduced non-specific binding and enhanced washing efficiency. The addition of Tween 20, a non-ionic detergent, helps to minimize background noise and improve assay sensitivity.

Three established applications of PBS with Tween 20 (PBST) pH 7.4 include:

• Western Blotting: Used extensively as a wash buffer to remove unbound antibodies and reduce background in immunodetection.
• ELISA (Enzyme-Linked Immunosorbent Assay): Crucial for washing steps between incubation periods, preventing non-specific binding and ensuring accurate signal detection.
• Immunohistochemistry (IHC) and Immunofluorescence (IF): Employed for washing tissue sections or cells to remove unbound antibodies and reduce background staining.

This PBS with Tween 20 (PBST) pH 7.4 solution from Canvax Biotech is manufactured under stringent quality control to ensure high purity and reliable performance in your experiments.