pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase.

References

• Impact of sunflower (Helianthus annuus L.) plastidial lipoyl synthases genes expression in glycerolipids composition of transgenic Arabidopsis plants. Nature Scientific Reports 2020.
• Functional characterization of VviDHN2 and VviDHN4 dehydrin isoforms from Vitis vinifera (L.): an in silico and in vitro approach. Plant Physiology and Biochemistry 2020.
• Synthesis of aromatic amino acids from 2G lignocellulosic substrates. Microbial Biotechnology 2021.
• Characterization and impact of sunflower plastidial octanoyltransferases (Helianthus annuus L.) on oil composition. Journal of Plant Physiology 2022.