For the Quick isolation of High-Quality Total RNA, DNA and/or Protein from a Single Sample

PRImeZOL™ Reagent is a ready-to-use reagent for the isolation of total RNA from various Biological materials such as Animal and Plant Tissues (rich in polysaccharides and proteoglycans), Cell culture and Bacterial cells.

This procedure is based on the sample lysis in cationic detergent guanidinium thiocyanate (GTC), followed by organic extractions and alcohol precipitation. The biological sample is homogenized or lysed before being separated into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA remains in the aqueous phase and its purification is followed by precipitation in isopropyl alcohol.

After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. PRImeZOL™ Reagent contains phenol and the mixture of other reagents to ensure optimal results.”

Advantages and Features

• Quick isolation of high-quality total RNA, DNA and/or protein from a single sample.
• Ready-to-use solution.
• Relevant Cost-saving.
• Proven performance with large or small amounts of tissue or cells.
• Versatile: ideal for a wide variety of biological materials such as animal and plant tissues (rich in polysaccharides and proteoglycans), cell culture and bacterial cells.
• Pure RNA: ideal for any downstream application such as RT-PCR, in vitro translation, Northern blotting, RNase protection assays or dot blot hybridization.

Applications

• Purified RNA is ideal for any downstream application such as RT-PCR, in vitro translation, Northern blotting, RNase protection assays or dot blot hybridization.
• Purified DNA can be used for PCR and Southern blotting.
• Purified protein can be used for Western blotting.

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References

• The antioxidant response favors Leishmania parasites survival, limits inflammation and reprograms the host cell metabolism. PLOS Pathogens 2021.
• cFLIP downregulation is an early event required for endoplasmic reticulum stress-induced apoptosis in tumor cells. Nature Cell Death and Disease 2022.
• Antioxidant Responses Induced by PFAS Exposure in Freshwater Fish in the Veneto Region. Preprints 2022
• MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/typeI IFN responses via Brd4 and is involved in ALS pathophysiology. PNAS 2023.
• Targeting the gut microbiota with dietary fibers:a novel approach to prevent the development cardiovascular complications linked to systemic lupus erythematosus in a preclinical study. Gut Microbes 2023.