This Tris-Glycine-SDS pH 8.3 buffer is a high-quality, ready-to-use solution for denaturing protein electrophoresis. Ideal for SDS-PAGE and Western blotting, it ensures reliable protein separation.
We can also custom manufacture this product from Research-grade to Clinical-grade under GMP basis on commercial scale.
Available formats: 1 L (1x), 1 L (10X)
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Tris-Glycine SDS pH 8.3 (TG-SDS) is a high-quality, reliable, and convenient buffer, ready for immediate use in your laboratory. This solution incorporates the powerful denaturing agent sodium dodecyl sulfate (SDS), making it an indispensable component for protein electrophoresis under denaturing conditions (SDS-PAGE).
SDS-PAGE involves separating proteins primarily based on their size. By treating samples under denaturing and reducing conditions with SDS, proteins efficiently unfold and become coated with SDS detergent molecules. This coating imparts a uniform negative charge-to-mass ratio, allowing proteins to migrate through the polyacrylamide gel primarily according to their molecular weight.
Key applications of Tris-Glycine SDS pH 8.3 include:
- Protein Electrophoresis: Essential as a running buffer for separating proteins under denaturing conditions, ensuring clear and consistent results.
- SDS-PAGE: Optimized for sodium dodecyl sulfate polyacrylamide gel electrophoresis, providing the necessary environment for efficient protein separation.
- Western Blotting: Crucial for subsequent protein transfer from gels to membranes in Western blotting procedures, facilitating accurate immunological detection.
