For Optimized, Accurate & Sensitive Colorimetric Detection of Cellular Metabolic Activities.
The XTT Cell Proliferation Assay Kit is a reliable, easy-to-use, and sensitive solution designed for quantifying cell proliferation and cytotoxicity in a wide range of experimental systems. This colorimetric assay enables researchers to evaluate the growth, viability, and metabolic activity of cultured cells with precision. Based on the reduction of tetrazolium salt XTT to a water-soluble formazan dye, the kit provides reproducible results for high throughput assays and routine applications in cell biology and drug discovery.
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For Optimized, Accurate & Sensitive Colorimetric Detection of Cellular Metabolic Activities
XTT Cell Proliferation Assay Kit
The XTT Cell Proliferation Assay Kit is a reliable, easy-to-use, and sensitive solution designed for quantifying cell proliferation and cytotoxicity in a wide range of experimental systems. This colorimetric assay enables researchers to evaluate the growth, viability, and metabolic activity of cultured cells with precision. Based on the reduction of tetrazolium salt XTT to a water-soluble formazan dye, the kit provides reproducible results for high throughput assays and routine applications in cell biology and drug discovery.
What Is a Cell Proliferation Assay?
A cell proliferation assay is a laboratory method to measure the expansion of viable cells in culture. These assays allow scientists to study how growth conditions, compounds, or growth factors influence the proliferation of a cell line or population. The XTT assay is one of the most widely used methods because it is simple, fast, and does not require solubilization steps to obtain a clear solution for analysis with an ELISA reader.
Principle of the XTT Assay
The XTT method is based on the ability of metabolically active cells to reduce the tetrazolium salt XTT into a soluble, orange-colored formazan product. The reaction involves electron transfer through mitochondrial enzymes. The quantity of the formazan dye produced is directly proportional to the number of living cells in the sample. Results are measured in a flat bottom microplate format using a microplate reader or ELISA reader.
Applications in Cell Biology
Researchers use the XTT kit for:
- Determination of cell proliferation in cancer and primary cell lines
- Cytotoxicity testing for drugs, natural compounds, and chemicals
- Evaluating cell viability in suspension and adherent cells
- Screening assays for inhibitors, activators, or growth factors
- Studies published in scientific reports, the Journal of Cell Biology, and related scientific communications
The kit provides versatility for both adherent and suspension cells, making it suitable for a wide variety of experimental needs.
Advantages of the XTT Cell Proliferation Assay Kit
- No solubilization step required to obtain a clear solution
- Compatible with both adherent and suspension cells
- Designed for high throughput assays in 96- or 384-well plates
- Highly reproducible results with minimal background interference
- Simplified protocol that is easy to perform
- Reliable quantification of cell proliferation and cytotoxicity
How the Assay Works
- Seed cells into a 96-well flat bottom culture plate.
- Add test agents, such as compounds, growth factors, or serum supplements.
- Incubate under controlled temperature and COâ‚‚ conditions.
- Prepare reagents according to the protocol and mix each vial thoroughly.
- Add the XTT solution and coupling reagent immediately to each well.
- Allow the reaction mixture to develop a colored product during incubation.
- Measure absorbance values with an ELISA reader.
This straightforward protocol ensures accuracy and consistency in determining the number of viable cells.
Key Features
- Sensitive detection of metabolically active cells
- Minimal hands-on time during setup
- Optimized for reproducibility and reliability
- Validated in multiple scientific studies and peer-reviewed journal publications
- Suitable for screening libraries of products and compounds
Compatibility with Cell Lines and Culture Systems
The XTT kit can be applied to a broad range of cell lines, including cancer cells, stem cells, and primary cells. Both adherent and suspension cells can be analyzed without modification to the workflow. Researchers working with tissue culture grade plastics and serum-supplemented medium will find the kit adaptable to standard laboratory setups.
Practical Considerations
- Always mix each vial thoroughly before preparing the reagent mixture.
- Add the coupling reagent immediately to ensure optimal reduction of tetrazolium salt.
- Avoid exposing reagents to light or inappropriate storage conditions.
Protocol Highlights
- Ready-to-use reagents
- Detailed instructions included
- Compatible with 96-well and 384-well plates
- Designed for reproducibility across multiple experiments
Following the protocol carefully allows you to measure cellular responses consistently.
Handling and Storage
- Store reagents at recommended temperature
- Protect from light exposure
- Stable for long-term use when stored correctly
Why Choose This Kit?
The XTT Cell Proliferation Assay Kit delivers robust and reproducible results, enabling scientists to perform accurate quantification of cellular responses. By combining ease of use, reliability, and scalability, the kit has become a standard tool for researchers studying cell proliferation and cytotoxicity.
References
- An ADAR1-dependent RNA editing event in the cyclin-dependent kinase CDK13 promotes thyroid cancer hallmarks. Molecular Cancer 2021.
| Weight | 0.1 kg |
|---|---|
| Dimensions | 10 × 10 × 10 cm |
| pack-size |
