Endo free plasmid midiprep magnetic kit for rapid, large-scale extraction of endotoxin-free plasmid DNA (≈200 μg) from 100 mL bacterial culture via silica-coated magnetic beads—no centrifugation needed.
Available as 20- and 50-prep kit sizes.
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Midiprep kit for the fast and simple purification of endotoxin-free plasmid DNA, he PuroMAG™ Endo-Free Plasmid MidiPrep Kit is designed for large-scale extraction from cultured bacterial cells, allowing you to purify plasmid DNA from up to 100 mL of overnight culture, with yields of up to 200 µg.
The purification technology is based on the binding of plasmids to silica-coated magnetic beads, which ensures efficient removal of genomic DNA, proteins, salts, and other contaminants. Following the lysis steps, endotoxins are specifically removed using a dedicated Endotoxin Removal Buffer (ERB), with final endotoxin levels of less than 0.1 EU/µg.
Key features of this kit include:
- Rapid Purification: The magnetic bead-based protocol allows for completion in under 60 minutes, significantly faster than traditional methods.
- No Centrifugation: The streamlined workflow eliminates the need for centrifugation, making it ideal for both manual and automated processes.
- High Purity: Optimized buffer formulations ensure efficient removal of RNA, resulting in highly pure plasmid DNA.
The extracted high-quality, endotoxin-free plasmid DNA is perfect for a wide range of sensitive downstream applications, such as:
- Transfection of sensitive cell lines
- Cloning
- Sequencing
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