Boost nucleic acid purification with Carrier RNA Lyophilized Powder. This yeast tRNA enhances binding to silica columns and reduces RNase degradation, ensuring optimal results for low copy nucleic acids.
Available format: 5 x 600 µg
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Carrier RNA Lyophilized Powder
This product contains transfer RNA (tRNA) from yeast, designed to significantly improve your nucleic acid purification protocols. It works by two primary mechanisms:
- Enhanced Binding: Carrier RNA enhances the binding of low copy nucleic acids to silica columns, improving recovery rates during purification.
- RNase Protection: It effectively reduces the RNase degradation of purified RNA through competitive inhibition, thereby protecting your valuable samples.
Established Applications:
Carrier RNA is a versatile tool widely used in molecular biology for various applications, including:
- Nucleic Acid Precipitation: Commonly used to enhance the precipitation of low-concentration DNA and RNA during alcohol precipitation steps.
- Improved Recovery in Purification: Essential for maximizing the yield and purity of nucleic acids, especially when working with scarce or dilute samples.
- Protection Against Degradation: Provides a protective environment for nucleic acids, safeguarding them from enzymatic degradation during handling and storage.
Example of Usage
For silica column-based RNA extraction (e.g. Qiagen-style protocol):
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Mix sample (e.g. 200 µL plasma) with lysis buffer.
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Add 3 µg of carrier RNA (e.g. 3 µL of 1 µg/µL solution).
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Continue with ethanol addition and binding to the column.
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Proceed with wash and elution steps per your standard protocol.
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