Capping B solution with acetonitrile, 2,6-lutidine, and N-methylimidazole (50:30:20) is used in oligonucleotide synthesis to activate acetic anhydride during the 5’-OH capping step.
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Capping B Solution – Acetonitrile : 2,6-Lutidine : N-Methylimidazole (50:30:20 v/v/v)
This Capping B reagent is a ready-to-use solution of acetonitrile, 2,6-lutidine, and N-methylimidazole, precisely mixed in a 50:30:20 volume ratio. It is formulated for use in the capping step of solid-phase oligonucleotide synthesis, where it serves as an activator for acetic anhydride (Capping Reagent A).
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Composition: Acetonitrile : 2,6-lutidine : N-methylimidazole (50:30:20 v/v/v)
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Function: Activates acetic anhydride for 5’-OH acetylation
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Application: Capping B solution for DNA/RNA synthesis
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Instrument Compatibility: Suitable for manual and automated synthesizers
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Formulation: Ready-to-use
This formulation provides a balanced mix of steric and nucleophilic components, making it widely compatible across oligonucleotide synthesis protocols. It is designed for high performance in both small-scale research and commercial oligo manufacturing environments.
