BrightMax Lambda DNA/HindIII marker with 8 distinct fragments (125–23,130 bp). Ideal for accurate DNA size determination in agarose gels. Supplied ready-to-load in 6X loading buffer.
Quantity: 50 µg, at 0.1 µg/µL
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BrightMax Lambda DNA/HindIII Ladder/Marker
The BrightMax Lambda DNA/HindIII Marker is a precise and highly stable DNA Molecular Weight Marker. It is ready-to-use and specifically designed for accurate sizing and quantification of double-stranded DNA fragments ranging from 125 bp to 23.1 kb. This marker contains loading dyes with one or two migration visualization dyes, providing convenience for your gel analysis.
Key Features:
- Ready-to-Use Convenience: This marker is supplied ready for immediate use, simplifying your experimental workflow.
- Broad Sizing Range: Suitable for DNA fragments from 125 bp up to 23.1 kb, ideal for large DNA analysis.
- Enhanced Visibility: Contains loading dyes with one or two migration visualization dyes, enabling clear monitoring of DNA migration during electrophoresis.
- Precise Bands: Composed of 8 distinct fragments, providing sharp and clear bands on the gel.
- Increased Intensity Bands: The 2.3 kb and 4.4 kb bands have increased intensity on an ethidium bromide-stained agarose gel, aiding in identification and orientation.
- Convenient Packaging: Provided in a convenient format containing 100 loads per vial, ensuring a long-lasting supply.
Applications:
The BrightMax Lambda DNA/HindIII Marker is a widely used and essential tool for researchers and clinicians performing nucleic acid gel analysis. It is particularly valuable for:
- Accurate Sizing of Large DNA Fragments: Precisely determine the size of DNA following restriction enzyme digestion, especially for larger constructs or genomic DNA.
- Restriction Fragment Length Polymorphism (RFLP) Analysis: Serves as a standard for RFLP studies where digested genomic DNA fragments need accurate size comparison.
- Southern Blotting: Frequently employed as a molecular weight standard when transferring large DNA fragments from gels to membranes for hybridization.
Contents:
The BrightMax Lambda DNA/HindIII Marker contains 8 individual DNA fragments at the following approximate sizes: 23.1, 9.4, 6.6, 4.4, 2.3, 2.0, 0.56, 0.125 kb. Most bands are supplied at approximately 40 ng/5 μl.
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