A complete, high-sensitivity reverse transcription system designed for converting total RNA or mRNA (from as little as 10 pg up to 5 µg) into full-length single-strand cDNA. The resulting cDNA is ideal for downstream applications such as RT-qPCR and RT-PCR. Carefully formulated to maximize yield, preserve RNA integrity, and boost sensitivity in gene expression assays.
Price:
Send Request
First Strand cDNA Synthesis Kit
Efficient & Sensitive complete System for First strand cDNA Synthesis from RNA templates
The First-Strand cDNA Synthesis Kit is an Efficient & Sensitive System for First strand cDNA Synthesis from RNA templates. It includes all the necessary components to synthesize first-strand cDNA, except the template RNA (total RNA or mRNA).
The First-Strand cDNA Synthesis Kit features two optimized mixes, RT Enzyme Mix and RT Reaction Mix. RT Enzyme Mix combines Reverse Transcriptase and RNase Inhibitor, while RT Reaction Mix contains dNTPs, oligo(dT)18 primers, random hexamers, MgCl2 and an optimized buffer. The synthesized single-stranded cDNA is suitable for real-time quantitative PCR applications. The Kit has been formulated to provide high yields of full-length cDNA product and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg to 5 μg of total RNA.
Advantages and Features
- Convenient: The kit includes all you need to synthesize cDNA from your total RNA.
- Highly Sensitive: full-length cDNA starting at 10 pg – 5 μg of total RNA, due to a novel RNase H treatment.
- Full-length first-strand cDNA up to 15kb.
- Optimized to increase sensitivity in RT-qPCR and RT-PCR assays.
- Optimal reaction temperature: 50 °C
- Avoids RNA template degradation.
Applications
• First Strand cDNA Synthesis for RT-PCR and RT-qPCR.
• Construction of full length cDNA Libraries.
• RNA Analysis.
Datasheet and cDNA synthesis protocol
References
- Potential spillover investigated by metagenome sequencing in Laikipia, Kenya reveals tick-borne pathogens and a novel bunyavirus. One Health 2025.
| Weight | 0.1 kg |
|---|---|
| Dimensions | 10 × 10 × 10 cm |
| pack-size |
