For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use

Product Overview:

pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency.

The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

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White Paper: Advantages of pSpark® over other popular DNA Cloning systems on the market: pGEM®-T and TOPO TA cloning®

Applications

• General cloning.
• Cloning of High Fidelity PCR amplified products.
• Production of ssDNA.
• Blue/white screening for recombinants.
• In vitro transcription from T7/SP6 dual-opposed promoters.

References

• Molecular and biochemical analysis of XDH from Phaseolus vulgaris suggest that uric acid protects the enzyme against the inhibitory effects of nitric oxide in nodules. Plant Physiology and Biochemistry 2019.