Optimizing Use of Your Desalting Column
How Desalting Columns Work Desalting columns use size exclusion chromatography (SEC). They may also be called gel filtration columns, size-exclusion columns, or buffer exchange columns. The resin inside contains porous beads, typically made of cross-linked dextran (e.g. Zetadex, or Sephadex). Large molecules like proteins or nucleic acids cannot enter the bead pores and elute quickly, … Read more







