CentriPure 2, a 200 µL Desalting Column

The CentriPure 2 desalting columns are pre-packed gravity-flow columns optimized for rapid desalting and buffer exchange of nucleic acids and proteins. Using size-exclusion chromatography, these columns remove low-molecular-weight substances such as salts, dyes, and unincorporated labels while retaining target macromolecules.

Features:

• Gravity-flow format

• MWCO: ~5 kDa (nucleic acids <10 bp)

• Resin: Zetadex®

• Pre-packed and ready to use

• Sold in packs of 50 columns

Applications:

• Desalting and buffer exchange of DNA, RNA, and proteins

• Removal of small molecules prior to enzymatic reactions or labeling

• Sample prep for downstream molecular biology workflows

Specifications:

• Column format: Z25M

• Sample volume: a 200 µL desalting column, for sample sizes 150-300 µL

• Flow method: gravity — no centrifuge required

CentriPure 2 columns offer consistent, high-quality purification with minimal dilution and fast processing, making them ideal for small-volume workflows.

Interested in testing CentriPure P2 columns?

References

• An oligomeric state‐dependent switch in the ER enzyme FICD regulates AMPylation and deAMPylation of BiP. The EMBO Journal 2019.
• Linker length affects photostability of protein-targeted sensor of cellular microviscosity. Marketa Kubankova et al 2019 Methods Appl. Fluoresc. in press
• Inhibiting Type VI Secretion System Activity with a Biomimetic Peptide Designed To Target the Baseplate Wedge Complex. mBio 2021.