The PuroSPIN™ Endo-Free Plasmid MIDIprep Kit is designed to provide a simple and easy-to-use method for large-scale extraction and purification of endotoxin-free plasmid DNA.
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Endotoxin Free Plasmid Midiprep Kit
The PuroSPIN™ Endo-Free Plasmid MIDIprep Kit is designed to provide a simple and easy-to-use method for large-scale extraction and purification of endotoxin-free plasmid DNA. This purification technology is based on plasmid binding to silica spin-columns, which allows for efficient removal of genomic DNA, protein, salt, and other contaminants. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.
The PuroSPIN™ Endotoxin Free Plasmid MIDIprep Kit can extract up to 200 µg of plasmid DNA with endotoxin levels to <0.1 EU/µg. Silica spin columns provide a faster, simpler, and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
The PuroSPIN™ buffer formulations produce highly purified plasmid DNA for downstream applications, such as transfection, enzymatic digestion, or PCR.
Features:
- Purify endotoxin-free plasmid DNA from large volumes of bacterial culture
- Rapid purification of plasmid DNA (< 60 minutes), compared to ion-exchange column formats (> 3 hours)
- Optimized buffer formulations to ensure efficient removal of RNA from plasmid samples
- Efficient removal of genomic DNA, protein, salt, and other contaminants
- High quality extracted plasmid DNA can be used for a range of downstream applications such as cloning, PCR, restriction enzyme digest, sequencing, transformation, or transfection
