CentriPure 1000, a 100 mL Desalting Column – Large-Scale Gravity-Flow Purification

The CentriPure 1000 is a pre-packed desalting column designed for fast and reliable purification of nucleic acids and proteins. Using gravity-flow gel filtration, it efficiently removes salts, dyes, unreacted labeling reagents, and other small molecules from biological samples. It can also be used for fast and efficient buffer exchange.

Features:

• Pre-packed gravity-flow column

• Contains Zetadex® size-exclusion matrix

• Molecular weight cutoff: ~5 kDa (nucleic acids <10 bp)

• Optimized for high-volume processing (up to 100 mL)

• No centrifugation or vacuum required

Applications:

• Desalting of proteins and nucleic acids

• Buffer exchange

• Sample cleanup for enzymatic reactions

• Removal of small molecules and contaminants

Specifications:

• Column format: Z25M

• MWCO: ~5 kDa

• Sample volume: 100 mL desalting column

• Resin: Zetadex

• Flow method: gravity

CentriPure 1000 offers a unique solution for researchers working with large-scale preparations who need efficient, ready-to-use desalting without centrifugation or complex setups.

References

• An oligomeric state‐dependent switch in the ER enzyme FICD regulates AMPylation and deAMPylation of BiP. The EMBO Journal 2019.
• Linker length affects photostability of protein-targeted sensor of cellular microviscosity. Marketa Kubankova et al 2019 Methods Appl. Fluoresc. in press