Working with proteins, nucleic acids, or other macromolecules often requires removing unwanted salts, detergents, dyes, or reaction byproducts. Standard dialysis can be slow and inconvenient. That’s why researchers rely on size-exclusion spin columns—fast, reliable tools for desalting and buffer exchange that preserve recovery of the valuable analyte.
CentriPure 25 is a 2.5 mL sample desalting column using gravity-flow for fast purification of DNA, RNA, or proteins in ~2.5 mL samples. Removes salts and small molecules efficiently. Supplied in 25 column packs.
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CentriPure 25, a 2.5 mL Sample Desalting Column
Many experiments require the cleanup of macromolecules before they can be used in sensitive downstream applications. For example, high concentrations of salt can interfere with enzymatic reactions, spectroscopy, or cell-based assays. After a labelling reaction, excess fluorescent dye must be removed to ensure accurate results. Similarly, proteins conjugated with cross-linkers need purification from unreacted reagent. And in almost every case, you want to desalt or exchange buffer without losing sample.
The CentriPure 25Â is a 2.5 mL desalting column working by gravity-flow, pre-packed with Zetadex size-exclusion resin. CentriPure columns are designed to separate large molecules from small ones in just a few minutes. Proteins, DNA, or RNA pass through the column matrix quickly, while small solutes like salts, detergents, dyes, and other low molecular weight compounds are retained.
CentriPure columns offer fast, reliable desalting and buffer exchange for nucleic acids and proteins, effectively removing low-molecular-weight components such as salts, dyes, or unincorporated labeling reagents.
Features:
- Gravity flow column
- Pre-packed with Zetadex resin
- Molecular weight cutoff: ~5 kDa (nucleic acids <10 base pairs)
- Supplied ready to use in a 25-pack
Applications:
- Desalting – remove salts from protein or nucleic acid samples
- Buffer exchange – replace one buffer with another rapidly and efficiently
- Dye removal – purify labeled biomolecules by separating them from unbound dye
- Cleanup of biological samples in molecular biology workflows
Specifications:
- Column format: Z25M
- Sample volume: 2.5 mL
- Flow type: gravity — no centrifuge or vacuum system required
The CentriPure 25 desalting column combines ease of use with 2.5 mL sample volume cleanup needs in nucleic acid and protein purification.
Interested in testing CentriPure P25 columns?
References
- The role of plant cell wall encapsulation and porosity in regulating lipolysis during the digestion of almond seeds. Food and Function.
- AMPylation targets the rate-limiting step of BiP’s ATPase cycle for its functional inactivation. eLife 2017.
- Binding of ferredoxin NADP+ oxidoreductase (FNR) to plant photosystem I. Biochimica et Biophysica Acta (BBA) – Bioenergetics.
- Photoinduced Bleaching in an Efficient Singlet Oxygen Photosensitizing Protein: Identifying a Culprit in the Flavin-Binding LOV-Based Protein SOPP3. Journal of Photochemistry and Photobiology A: Chemistry 2022.
- Biocatalytic Synthesis of 2′-Deoxynucleotide 5′-Triphosphates from Bacterial Genomic DNA: Proof of Principle. Biotechnology and Bioengineering 2023.
- Designing a locally produced DENV-1 nucleic acid diagnostic for low resource regions with endemic disease. Analytical Methods 2025.
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