CentriPure 5, a 0.5 mL Desalting Column – Small-Volume Gravity-Flow Purification

The CentriPure 5 desalting column is a gravity-flow column pre-packed with Zetadex® size-exclusion resin. It allows rapid desalting and buffer exchange for proteins, oligonucleotides, and other biomolecules, while efficiently removing low-molecular-weight contaminants like salts and unreacted labels.

Features:

• Gravity-flow format

• Pre-packed with Zetadex resin

• MWCO: ~5 kDa (nucleic acids <10 bp)

• Supplied ready to use

Applications:

• Desalting and buffer exchange of nucleic acids and proteins

• Removal of small molecules such as dyes or unreacted reagents

• Sample cleanup prior to enzymatic reactions, synthesis, or labeling

Specifications:

• Column format: Z25M

• Sample volume: small-scale applications, 0.5 mL desalting column

• Flow method: gravity — no centrifugation needed

The CentriPure 5 desalting column delivers fast and reproducible sample cleanup with minimal hands-on time, making it ideal for low-throughput lab workflows requiring consistent results.

Interested in testing CentriPure P5 columns?

References

• Current approaches for purification of antibody-drug conjugates. Journal of Separation Science 2021.
• In Vitro Oxidative Crosslinking of Recombinant Barnacle Cyprid Cement Gland Proteins. Marine Biotechnology 2021.
• Photoinduced Bleaching in an Efficient Singlet Oxygen Photosensitizing Protein: Identifying a Culprit in the Flavin-Binding LOV-Based Protein SOPP3. Journal of Photochemistry and Photobiology A: Chemistry 2022.
• AJICAP Second Generation: Improved Chemical Site-Specific Conjugation Technology for Antibody-Drug Conjugate Production. ChemRxiv 2023.
• Discovery and optimization of a broadly-neutralizing human monoclonal antibody against long-chain α-neurotoxins from snakes. Nature Communications 2023.
• AJICAP Second Generation: Improved Chemical Site-Specific Conjugation Technology for Antibody–Drug Conjugate Production. Bioconjugate Chemistry 2023.