Gel Extraction Kit

PuroSPIN™ Gel Extraction Kit is designed to provide simple and easy-to-use method for extraction and purification of DNA samples (100 bp − 10 kbp) from agarose and PAGE gels. The purification technology is based on dissolving the gel embedded DNA in a buffer solution, followed by nucleic acid binding to silica spin-columns, which allows efficient removal of gel residues and other contaminants. Silica spin columns offer faster, simpler and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction. PuroSPIN™ buffer formulations produce highly purified DNA product for downstream applications, such as PCR, restriction digestion, ligation, labeling, or sequencing.

PuroSPIN™ columns can bind up to 200 μg of DNA product.

Features:

• Rapid purification of DNA fragments from standard agarose gels run in either TAE or TBE buffer
• Simple extraction process with no need for phenol or chloroform
• Efficient removal of dNTPs, primers, enzymes, and salts from PCR reaction mixtures and gel mixtures
• High quality extracted DNA can be used for a range of downstream applications such as sequencing, ligation, transformation, transfection, Southern blotting, qPCR, or microarray analysis